The Moores Cancer Center is one of just 55 NCI-designated Comprehensive Cancer Centers in the United States and the only one in San Diego County. As a consortium cancer center, it is a collaborative partnership between the UCSD: encompassing 14 departments (12 clinical, 2 basic sciences), 3 professional schools (School of Medicine, Skaggs School of Pharmacy and Pharmaceutical Sciences, School of Public Health), UCSD Health oncology hospitals and clinics; the basic and public health research and outreach of San Diego State University, and the basic and translational research of the LaJolla Institute of Immunology. These various programs and units are all dedicated to fulfilling the Moores Cancer Center's mission of reducing cancer's burden. As such, it ranks among the top centers in the nation conducting the continuum of cancer research, including ground-breaking scientific discovery, innovative translational research and clinical trials, providing advanced level cancer care, and serving the community through outreach and education programs.

As a top-ranking, future-oriented organization, we offer challenging career opportunities in a fast-paced and innovative environment. Moores Cancer Center Business Office follows a progressive philosophy of career-path development for its employees including opportunities for cross-training, professional development, and progressive responsibility.

The Simeone lab, with 25 years of continuous NIH funding, has been driven to understand the molecular determinants that drive pancreatic cancer development and progression. The lab works to define therapeutic vulnerabilities and uses laboratory-based models to study approaches for early detection, prevention, and development of novel therapeutics for pancreatic cancer. Specific areas of research interest are the DNA damage response and therapeutic vulnerabilities, understanding the complexities of the tumor microenvironment and mechanisms that drive therapeutic resistance, and developing new therapeutics to more effectively target pancreatic cancer.

Under general direction of the Principal Investigator, the Lab Manager will have dual responsibilities of overseeing and helping to drive the scientific direction and research productivity of the lab. The Lab Manager will serve as a senior scientist making significant innovative contributions pertaining to the development and design of new projects, grant writing, and establishing research collaborations between the Simeone lab and others across the UCSD community and beyond. For the right applicant, this position has the potential to lead to independent funding in the future.

Anticipated scientific duties will include partnering with the principal investigator in outlining and refining the strategic directions of the laboratory. Assist with project design and oversight, and supervision of lab personnel. Assisting in the preparation of data for publication and grant writing.

• Advanced theoretical knowledge and/or Bachelor's Degree in cancer biology with a background in cancer biology, immunology, DNA damage response signaling.

• Experience independently isolating bone marrow and blood cells for analysis by flow cytometry, immunoblotting, gene expression and cell adhesion assays.

• Experience independently performing and directing in vivo experiments including the injection of growth factors, tumor cells and therapeutics, bleeding and tissue harvesting of mice and in performing minor surgery.

• Experience and willingness to work with laboratory animals.

• Experience independently performing and directing protein analysis techniques including protein determination, ELISA and Magpix assays and protein separation techniques.

• Experience independently working with endothelial, myeloid and tumor cell culture, DNA and RNA isolation from culture cells and from tissues, immunoblotting and quantitative RT-PCR for genes and proteins that regulate myeloid cell adhesion, migration and gene and protein expression in vitro and in vivo.

• Experience independently developing improvements to tissue culture techniques, including sterile technique, cell adhesion and migration assays.

• Experience performing independently performing and directing molecular biological techniques, including DNA purification, genotyping and cloning.

• Ability to develop new experimental procedures with accuracy and efficiency and to teach others to perform them with accuracy.

• Ability to use basic and advanced laboratory equipment, including centrifuges, spectrophotometers, balances pH meters, electrophoresis, single cell sequencing, magnetic and light based plate readers and flow cytometers equipment.

• Ability to write and follow step wise experimental protocols.

• High level organizational, analytical, and problem-solving skills.

• Willingness and ability to handle mice for surgical procedures, including anesthesia administration. Experience to maintain animal colony by breeding, genotyping and weaning animals, creating new animal strains and keeping accurate records.

• Strong communication skills (both verbal and written) needed to interact professionally and effectively in the work environment, as well as to effectively and accurately document research procedures. Ability to read, comprehend, and discuss research materials. Grant and manuscript writing experience.

• Ability to adhere to established UCSD guidelines for safety and environmental issues.

• Experience with personal computers, including word processing and spreadsheets. Ability to use Quartzy and Oracle to place and reconcile orders and receipts.

• Ability to maintain confidentiality. Demonstrated administrative experience, skill to coordinate complex activities and to use independent judgment to organize and prioritize laboratory functions.

• Experience with laboratory management duties, including training and directing student employees and volunteers, ordering and maintaining inventory, organizing and maintaining freezers, cold room, and equipment.

• Knowledge to manage official travel and entertainment documentation and file reimbursements requests

• Interest and ability to teach students, postdocs and new personnel methods and skills

• Demonstrated knowledge in making innovative contributions to experimental design and methodology by reviewing

• Demonstrated ability use and adapt existing techniques and technologies in novel and non-traditional ways in order tomeet research objectives.

• PhD and post-doctoral training with at least 5 years lab experience in cancer biology with a background in cancer biology, immunology, DNA damage response signaling

• Enthusiasm for learning and a genuine interest in scientific research. Willingness and ability to learn new skills, research methods, and scientific principles. Experience and interest in developing novel techniques and analytical methods.

• Employment is subject to a criminal background check.

• Must be willing to work frequently with animals and to work occasional nights and weekends.

• Proven ability to work with animals and willingness to work with biohazardous materials.